Molecular Characterisation of Clostridium perfringens Type D Isolated from Sheep in Kashmir Himalayas, India

Isolation, molecular characterization and prevalence of Clostridium perfringens in sheep and goats of Kashmir Himalayas, India

Aim The study was conducted to report the occurrence of the Clostridium perfringens in sheep and goats of the Kashmir valley for the 1st time and to characterize them molecularly with respect to toxin genes to determine the prevalence of the various toxinotypes. Materials and Methods A total of 177 samples (152 from sheep and 25 from goats) collected from healthy, diarrheic animals, and morbi...

Molecular typing of toxigenic Clostridum perfringens isolated from sheep in Iran

In this research a molecular method based on polymerase chain reaction for typing of Clostridium perfringens was developed and toxin genotypes of 64 isolates from sheep and goats in Iran were determined. The PCR assays were developed for detection of alpha (cpa), beta (cpb) and epsilon (etx) toxin genes, allowing classification of the isolates into genotypes A B, C and D. The field isolates ...

molecular typing of clostridium perfringens isolated from minced meat

the aim of this study was to determine the presence of toxin genes in clostridium perfringens isolated from 200minced meat samples using molecular typing. for this purpose, after isolation of clostridium perfringens using conventional culture method and confirmation by specific 16srd na gene pcr, alpha (cpa), beta (cpb), beta 2 (cpb2),epsilon (etx), iota (ia) and enterotoxin (cpe) genes were in...

Preparation of Standard Clostridium perfringens Antitoxin in the Sheep

Genotyping of Clostridium perfringens isolated from broiler meat in northeastern of Iran

Clostridium perfringens is an important cause of bacterial food poisoning worldwide. The disease is caused by C. perfringens enterotoxin (CPE) encoded by cpe gene. The aim of this research was to identify the different types of C. perfringens and the presence of cpe gene in isolated bacteria from broilers’ meat marketed in retail meat shops of Mashha...